Patterns of Cryptosporidium antigen and oocyst excretion in calves studied by reverse passive haemagglutination and...

A reverse passive haemagglutination (RPH) assay incorporating a monoclonal antibody against Cryptosporidium parvum oocysts was used to follow Cryptosporidium coproantigen excretion by calves. RPH detected soluble antigen that passed 0.22 micron filters. Non-specific reactions that occurred in some samples were markedly reduced by heat treatment of the faecal specimens and were abolished by filtration after heat treatment. Results were compared with oocyst counts performed by microscopy of modified Ziehl-Neelsen (MZN) stained faecal smears. Five hundred and thirty-two daily specimens were examined from 30 calves. The mean age at which positive results for both oocysts and antigen was detected was 9 days (range 5-15 days), and excretion lasted for 5-11 days with some cycling of positive reactions in some calves. The occasional cycling to a negative reaction demonstrates a need to take samples from consecutive days to ensure diagnosis. Two hundred and ninety-one (54.7%) specimens were negative in both tests, 178 (33.5%) were positive in both, 14 (2.6%) were positive only by microscopy, and 49 (9.2%) were positive only by RPH. By these criteria the kappa coefficient of agreement between the tests was good (0.753). Compared with MZN, the sensitivity of RPH is 92.7%, specificity 85.6%, positive predictive value 78.4% and negative predictive value 95.4%. The method is simple, objective, has ease of quality control, and either single samples or batches can be processed.