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Related Experiment Videos

Attomole-sensitivity electrospray source for large-molecule mass spectrometry

G A Valaskovic1, N L Kelleher, D P Little

  • 1Baker Chemistry Laboratory, Cornell University, Ithaca, New York 14853-1301, USA.

Analytical Chemistry
|October 15, 1995
PubMed
Summary

A novel electrospray ionization system coupled with Fourier transform mass spectrometry enables high-resolution mass spectra analysis of large biomolecules from minimal sample volumes. This advancement significantly reduces sample consumption for sensitive biomolecular characterization.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Mass Spectrometry

Background:

  • Characterizing large biomolecules like proteins and nucleotides requires sensitive analytical techniques.
  • Traditional mass spectrometry methods often demand larger sample volumes, limiting analysis of scarce samples.

Purpose of the Study:

  • To develop a new electrospray ionization (ESI) system for high-resolution mass spectrometry.
  • To enable sensitive analysis of large biomolecules (> 10 kDa) using minimal sample volumes.

Main Methods:

  • Fabrication of specialized ESI needles from fused-silica tubing.
  • Integration of the novel ESI system with Fourier transform mass spectrometry.
  • Analysis of large nucleotides and proteins using femtoliter sample volumes.

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Main Results:

  • Obtained full mass spectra with high resolving power (> 10^5).
  • Achieved high signal-to-noise ratios (400:1 at 8.6 fmol, 60:1 at 216 amol).
  • Demonstrated ultra-low sample consumption (approx. 10-150 amol).

Conclusions:

  • The new ESI system coupled with FT-MS provides highly sensitive and high-resolution analysis of large biomolecules.
  • This method is effective for analyzing minute quantities of proteins and nucleotides.
  • The technique significantly advances the capability for characterizing precious or limited biological samples.