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A new plasmid-encoded proteic killer gene system: cloning, sequencing, and analyzing hig locus of plasmid Rts1
Q B Tian1, M Ohnishi, A Tabuchi
1Department of Bacteriology, Shinshu University School of Medicine, Japan.
Biochemical and Biophysical Research Communications
|March 18, 1996
Abstract:
A new proteic killer gene system, hig, was identified on the plasmid Rts1. The hig locus consisting of a higA and higB is directly related to the temperature sensitive host cell growth conferred by Rts1. We proved that higB encoding presumably a 92-amino-acid polypeptide inhibited segregation of plasmid free cells, and higA encoding a 104-amino-acid polypeptide suppressed the higB function both in cis and in trans.