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Determination of alternariol and alternariol methyl ether in apple juice using solid-phase extraction and high
T Delgado1, C Gómez-Cordovés, P M Scott
1Instituto Fermentaciones Industriales, Consejo Superior de Investigaciones Científicas (C.S.I.C.), Madrid, Spain.
Journal of Chromatography. A
|April 19, 1996
Abstract:
The present work describes a new method for determination of alternariol (AOH) and alternariol methyl ether (AME) in apple juice using solid-phase extraction (SPE) columns for extraction and cleanup of samples for high-performance liquid chromatography (HPLC). Chromatograms of spiked samples show that both toxins can be easily detected without interferences, and good recoveries for AOH (82.8 +/- 7.4%) and AME (91.9 +/- 6.1%) with detection limits as low as 1.6 and 0.7 micrograms/l, respectively, were obtained.