Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cold-induced hemolysis in a hypertonic milieu

F A Green, C Y Jung

    The Journal of Membrane Biology
    |May 12, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Cold temperatures induce red blood cell (RBC) hemolysis in hypertonic solutions. Membrane lipid phase transitions, influenced by hypertonic conditions and protein alterations, are implicated in this cold-induced RBC damage.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Pictorial review: A Step-by-step Guide to Coronary CT Angiography with Photon-Counting Detector CT.

    The British journal of radiology·2026
    Same author

    Association between Fiber Intake and Risk of Incident Chronic Kidney Disease: The UK Biobank Study.

    The journal of nutrition, health & aging·2023
    Same author

    Full-step Class II Correction Using a Modified C-palatal Plate for Total Arch Distalization in an Adolescent.

    The Journal of clinical pediatric dentistry·2018
    Same author

    Permeability of bimolecular membranes made from lipid extracts of human red cell ghosts to sugars.

    The Journal of membrane biology·2013
    Same author

    Peripheral glutamate receptors participate in interleukin-1beta-induced mechanical allodynia in the orofacial area of rats.

    Neuroscience letters·2004
    Same author

    Central cyclooxygenase-2 participates in interleukin-1 beta-induced hyperalgesia in the orofacial formalin test of freely moving rats.

    Neuroscience letters·2003

    Area of Science:

    • Biophysics
    • Cell Biology
    • Hematology

    Background:

    • Human erythrocytes (red blood cells) are susceptible to hemolysis under specific environmental conditions.
    • Hypertonic environments and temperature changes can significantly impact cell membrane integrity.

    Purpose of the Study:

    • To investigate the mechanism of cold-induced hemolysis in human erythrocytes suspended in hypertonic solutions.
    • To explore the role of membrane lipid phase transitions and protein alterations in this phenomenon.

    Main Methods:

    • Incubation of erythrocytes in hypertonic sodium chloride and sucrose solutions at varying temperatures (37°C to 0°C).
    • Analysis of membrane components, including phospholipids, cholesterol, and proteins, using techniques like gel electrophoresis.
    • Correlation of experimental findings with existing literature on temperature-induced membrane phase transitions.

    Related Experiment Videos

    Main Results:

    • Hemolysis occurred in two stages: initial incubation in hypertonic solute and subsequent cooling to 0°C.
    • Cooling rate was not critical, but ions reduced cold-induced hemolysis in hypertonic sucrose.
    • No significant release of membrane lipids; alterations observed in solubilized membrane proteins, with spectrin not identified.
    • Evidence suggests temperature-induced phase transitions in membrane lipids are involved.

    Conclusions:

    • Cold-induced hypertonic hemolysis is linked to temperature-induced phase transitions in erythrocyte membrane lipids.
    • Hypertonic conditions may cause membrane protein damage, hindering adaptation to lipid phase changes during cooling.