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Related Experiment Videos

Bleomycin resistance in Staphylococcus aureus clinical isolates

D Gennimata1, J Davies, A S Tsiftsoglou

  • 1Department of Pharmaceutical Sciences, Aristotle University of Thessaloniki, Greece.

The Journal of Antimicrobial Chemotherapy
|January 1, 1996
PubMed
Summary

Many Staphylococcus aureus clinical isolates exhibit resistance to the antibiotic bleomycin, often linked to tobramycin resistance and plasmid presence. Studies identified specific DNA sequences and bleomycin-binding properties contributing to this resistance in S. aureus.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Antibiotic resistance in Staphylococcus aureus is a growing clinical concern.
  • Bleomycin is an antibiotic used to treat certain cancers, but resistance can limit its efficacy.
  • Plasmids are known to carry genes conferring antibiotic resistance in bacteria.

Purpose of the Study:

  • To investigate the genetic basis of bleomycin resistance in clinical isolates of Staphylococcus aureus.
  • To identify specific DNA sequences and mechanisms responsible for bleomycin resistance.
  • To explore the association between bleomycin resistance and other antibiotic resistances.

Main Methods:

  • Testing clinical Staphylococcus aureus isolates for resistance to bleomycin and tobramycin.

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  • DNA dot-blot hybridization using a probe from plasmid pUB110 to detect resistance genes.
  • Analysis of bacterial cell lysates for bleomycin-binding properties (BBP).
  • DNA gel electrophoresis and Southern blot hybridization for detailed genetic analysis.

    • Main Results:

    • 197 clinical isolates of Staphylococcus aureus were found to be resistant to bleomycin.
    • Most bleomycin-resistant isolates were also resistant to tobramycin and contained plasmids.
    • 43 isolates (22%) carried pUB110-like bleomycin resistance DNA sequences.
    • Many resistant isolates exhibited bleomycin-binding properties (BBP) that protect bacterial DNA.
    • Six out of 13 analyzed strains carried pUB110-like bleomycin resistance DNA sequences.

    Conclusions:

    • Multiple genetic determinants may contribute to bleomycin resistance in Staphylococcus aureus.
    • The presence of pUB110-like sequences and bleomycin-binding properties are significant factors in S. aureus bleomycin resistance.
    • Further research is needed to fully elucidate the genetic mechanisms of bleomycin resistance in S. aureus.