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Related Experiment Videos

Characterization of murine intercellular adhesion molecule-2

H Xu1, J K Bickford, E Luther

  • 1Department of Molecular Genetics and Protein Chemistry, Pfizer Central Research, Groton, CT 06340, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|June 15, 1996
PubMed
Summary

Researchers developed rat monoclonal antibodies against murine intercellular adhesion molecule-2 (ICAM-2). This study found ICAM-2 is conserved across species, with similar function in mice and humans.

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Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Intercellular Adhesion Molecule-2 (ICAM-2) is a cell surface glycoprotein involved in immune cell interactions.
  • Understanding ICAM-2's function and expression is crucial for comprehending immune responses.

Purpose of the Study:

  • To generate rat monoclonal antibodies (mAbs) against murine ICAM-2.
  • To characterize the murine ICAM-2 glycoprotein and its expression patterns.
  • To investigate the functional role of murine ICAM-2 in cell adhesion.

Main Methods:

  • Raising rat monoclonal antibodies against murine ICAM-2.
  • Immune precipitation and purification to determine protein size.
  • Flow cytometry or similar techniques to assess ICAM-2 expression on various cell lines.

Related Experiment Videos

  • Inhibition assays using mAbs to study ICAM-2 and LFA-1 interactions.

    • Main Results:

    • Murine ICAM-2 is a 55 kDa glycoprotein, similar in size to human ICAM-2.
    • ICAM-2 is expressed on diverse leukocyte cell lines (T lymphoma, B lymphoma, mastocytoma, macrophages) and endothelioma cells.
    • Unlike ICAM-1, ICAM-2 expression is not upregulated by inflammatory cytokines.
    • Specific anti-ICAM-2 mAbs inhibited the binding between LFA-1 and ICAM-2.

    Conclusions:

    • Murine ICAM-2 shares structural and functional similarities with human ICAM-2.
    • The findings suggest functional conservation of ICAM-2 across species.
    • ICAM-2 plays a role in LFA-1-mediated cell adhesion.