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Luminal exposure of oxidants alter colonic absorptive function
L Bhaskar1, K A Balasubramanian
1Wellcome Research Laboratory, Department of Gastrointestinal Sciences, Christian Medical College Hospital, Vellore, India.
Life Sciences
|January 1, 1996
Summary
Colonic oxidants trigger fluid and electrolyte secretion by stimulating prostaglandin generation, not by damaging mucosal lipids. This suggests a prostaglandin-mediated mechanism for oxidant-induced colonic dysfunction.
Area of Science:
- Gastroenterology
- Cellular Biology
- Toxicology
Background:
- The colonic lumen contains various oxidants from diet and bacterial metabolism.
- These oxidants may impact colonic mucosal function and integrity.
Purpose of the Study:
- To investigate the effects of luminal oxidants on colonic mucosal lipid peroxidation and absorptive function.
- To determine the role of prostaglandin generation in oxidant-induced colonic changes.
Main Methods:
- Exposure of the colon to different luminal oxidants.
- Measurement of colonic mucosal lipid peroxidation.
- Assessment of fluid and electrolyte secretion.
- Use of indomethacin, a prostaglandin synthesis inhibitor, as a control.
Main Results:
- All tested oxidants induced fluid and electrolyte secretion.
- Indomethacin reversed the oxidant-induced secretion.
- Oxidants did not cause significant lipid peroxidation in the colonic mucosa.
Conclusions:
- Luminal oxidants alter colonic function, primarily through prostaglandin generation.
- Oxidant-induced colonic dysfunction occurs independently of significant mucosal lipid peroxidation.