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The v-erbA oncogene selectively inhibits iodide uptake in rat thyroid cells
F Trapasso1, M L Martelli, C Battaglia
1Dipartimento di Medicina Sperimentale e Clinica, Facoltà di Medicina eChirurgia di Cantanzaro, Università degli Studi di Reggio Calabria, Italy.
Abstract:
v-erbA is the oncogenic form of the c-erbA proto-oncogene, which encodes the receptor for thyroid hormones. The expression of the v-erbA oncogene in thyroid differentiated cells, PC Cl 3, inhibits iodide uptake and thyrotropin-dependent growth, whereas it has no effect on the expression of the other thyroid specific markers, i.e. thyroglobulin, thyroperoxidase and thyrotropin receptor. The activity of transcription factor AP-1, evaluated by a specific DNA binding assay and by transcription of AP-induced promoter (TRE) is enhanced in PC v-erbA cells. v-erbA mutants in the DNA binding domain do not affect the iodide uptake of thyroid cells nor AP-1 activity. We suggest that this transcriptional activation mediates the selective effects of v-erbA on the expression of thyroid specific markers.