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Quantitative evaluation of fine-needle aspiration
C R Cannon1, L D Richardson, W Replogle
1Department of Surgery, University of Mississippi School of Medicine, Jackson, USA.
Summary
This study optimized lymphocyte extraction from tonsils using fine-needle aspiration. A 20-cm3 syringe and 21-gauge needle provided the highest cell yield for diagnostic purposes.
Area of Science:
- Immunology
- Cell Biology
- Medical Diagnostics
Background:
- Lymphocyte isolation from tonsillar tissue is crucial for immunological studies and diagnostics.
- Optimizing cell yield and viability is essential for accurate analysis.
Purpose of the Study:
- To develop and evaluate a protocol for obtaining mature lymphocytes from freshly harvested tonsils.
- To determine the optimal combination of syringe and needle size for lymphocyte extraction.
Main Methods:
- A protocol involving various syringe (5, 10, 20 cm3) and needle (21, 23, 27 gauge) sizes was tested.
- Cells were suspended in Earle's balanced salt solution and counted using an automated cell counter.
- Negative pressure was measured using a manometer for each syringe/needle combination.
Main Results:
- Cell counts ranged from 900 to 2800 cells/mm3, providing adequate material for diagnostics.
- Negative pressures ranged from -500 to -700 cm of H2O.
- Fine-needle aspiration using a 20-cm3 syringe and a 21-gauge needle yielded the best results.
Conclusions:
- The developed protocol effectively isolates mature lymphocytes from tonsils.
- Specific syringe and needle dimensions are critical for maximizing cell yield.
- The 20-cm3 syringe with a 21-gauge needle is recommended for optimal tonsillar lymphocyte extraction.