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[Chronic lymphatic leukemia from B CD5+ cells: characteristics, clinical and laboratory features, and
1Kliniki Hematologii AM w Warszawie.
Insights
This study on chronic lymphocytic leukemia B-cell (CLL B) revealed a negative correlation between CD5+ B cells and lifespan. Enhanced T-cell lymphocytosis was observed, with correlations to disease progression.
Area of Science:
- Hematology
- Immunology
- Oncology
Background:
- Chronic lymphocytic leukemia B-cell (CLL B) is a lymphoid malignancy.
- Understanding the immunological phenotype is crucial for prognosis.
Purpose of the Study:
- To investigate the immunological phenotype of peripheral blood lymphocytes in CLL B patients.
- To explore correlations between specific lymphocyte markers and clinical parameters.
Main Methods:
- Studied 54 CLL B cases (1990-1993).
- Determined immunological phenotype of peripheral blood lymphocytes using flow cytometry.
- Analyzed correlations between lymphocyte markers (CD5+, CD19+, CD23+, etc.) and clinical data (lymphadenopathy, splenomegaly, lifespan, disease bulk).
Main Results:
- 100% of patients had CD5+ B cells; 70% had sIg+ lymphocytes.
- Negative correlation found between CD5+ B cells and lifespan (p < 0.03).
- Positive correlation observed between CD23 expression and bulky disease (p < 0.01).
- Diminished T cells (CD2+, CD3+, CD4+, CD8+) but enhanced T-cell lymphocytosis with these markers.
- Positive correlations between T-cell lymphocytosis markers (CD2+, CD4+, CD8+) and blood/bone marrow lymphocytosis.
Conclusions:
- The immunological profile of CLL B involves characteristic B and T cell alterations.
- CD5+ B cell expression is inversely related to patient survival.
- Specific T-cell lymphocytosis markers correlate with disease burden and extent.
Abstract:
Fifty four cases of CLLB were studied from 1st of April. 1990 to October 30, 1993; 35 male and 19 female (M:F = 1.8:1) in age 39-76 years (median age = 62 years). 81% patients had lymphadenopathy, 30%--hepatomegaly, 31% splenomegaly, 24% had allergic symptoms, 24% had anaemia (7% AINH). 13% thrombopoenia (2% autoimmunologic thrombopoenia). In all cases immunological phenotype of peripheral blood lymphocytes was determined, 100% patients had B cells CD5+, 70% lymphocytes sIg+, 97%-CD19+, 73%-CD23+, 67%-CD22+, 82%-HLADr, 10%-71(TR90), CD10 was negative. There was negative correlation between B CD5+ cells and life span (p < 0.03). There was positive correlation, between CD23 and bulky diseases (p < 0.01). Percentage of T cells with CD2+, CD3+, CD4+, CD8+ and CD4:CD8 was diminished. Lymphocytosis T with antigens CD2+, CD3+, CD4+, CD8+ was enhanced. There was found a positive correlation between lymphocytosis CD2+ (p < 0.00009), CD4+ (p < 0.008), CD8+ (p < 0.0008) and blood lymphocytosis and positive correlation between T lymphocytosis CD2+ (p < 0.02), CD4+ (p < 0.002) and lymphocytosis bone marrow.