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Gene mapping by chromosome microdissection and microisolation in the chicken
E M Shaw1, J S Otis, S J Lamont
1Department of Animal Science, University of Minnesota, St. Paul 55108, USA.
Poultry Science
|January 1, 1996
Summary
This study introduces a new method for pinpointing chicken gene locations on chromosomes using chromosome microdissection and filter hybridization. This technique aids in mapping cloned genes, offering an alternative to existing hybridization methods.
Area of Science:
- Genetics
- Molecular Biology
- Cytogenetics
Background:
- Accurate chromosomal localization of cloned genes is crucial for understanding genome organization and function.
- Existing methods like in situ hybridization have limitations in resolution and application.
Purpose of the Study:
- To develop and validate a novel technique for chromosomal localization of cloned chicken genes.
- To establish chromosome microdissection and microisolation as a viable method for gene mapping.
Main Methods:
- Chromosome microdissection and microisolation from metaphase spreads.
- DNA amplification using polymerase chain reaction (PCR).
- Dot blot analysis with biotinylated DNA probes for gene detection.
Main Results:
- Successfully localized the chicken MHC gene to the nucleolar chromosome and the beta-actin gene to chromosome 2q.
- Demonstrated the efficacy of the developed technique using these known genes as markers.
Conclusions:
- Chromosome microdissection and microisolation combined with filter hybridization is an effective method for cloned gene localization.
- This technique serves as a valuable complementary or alternative approach to in situ hybridization methods for gene mapping in chickens.