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The cellular dermal infiltrate in experimental immediate type cutaneous hypersensitivity
F Sjögren1, C Anderson, O Groth
1Department of Dermatology, University Hospital, Linköping, Sweden.
Acta Dermato-Venereologica
|November 1, 1995
Summary
This study adapted a guinea pig model to analyze immediate intradermal reactions to ovalbumin. The research identified a two-phase inflammatory response, with neutrophils in the early phase and mononuclear cells and basophils in the late phase.
Area of Science:
- Dermatology
- Immunology
- Allergy Research
Background:
- A guinea pig model previously studied dermal inflammatory responses to allergic, toxic, and irritant reactions.
- This model was adapted to investigate immediate intradermal reactions to ovalbumin.
Purpose of the Study:
- To characterize the dermal inflammatory cell infiltrate in an immediate intradermal ovalbumin reaction in guinea pigs.
- To establish reliable methods for quantifying cellular infiltration in this model.
Main Methods:
- Adapted a guinea pig model for immediate intradermal ovalbumin reactions.
- Quantified and compared infiltrating cells at three dermal levels.
- Focused on counting 20 subepidermal fields from the allergen injection site for reliable data.
Main Results:
- The reaction exhibited two distinct phases microscopically.
- The early phase showed a high proportion of neutrophils.
- The late phase was characterized by a predominance of mononuclear cells and basophil granulocytes, with some similarities to delayed allergic contact reactions.
Conclusions:
- The adapted guinea pig model reliably characterizes the biphasic dermal inflammatory response to intradermal ovalbumin.
- The early and late phases of this reaction exhibit distinct cellular profiles, with the late phase showing basophil predominance.