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Related Experiment Videos

Lysosomal enzymuria in preeclampsia

D W Jackson1, A Sciscione, T L Hartley

  • 1Department of Biochemistry, University of New Mexico, School of Medicine, Albuquerque, NM 87131 USA.

American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation
|June 1, 1996
PubMed
Summary

Preeclampsia is linked to kidney tubule injury, altering lysosomal enzyme levels. Increased urinary enzyme excretion may serve as a diagnostic marker for preeclampsia in pregnant women.

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Area of Science:

  • Nephrology
  • Biochemistry
  • Obstetrics

Background:

  • Preeclampsia is a pregnancy complication potentially involving kidney damage.
  • Lysosomal enzymes may be released due to proximal tubule epithelial injury in preeclampsia.
  • Investigating these enzymes could offer diagnostic or predictive markers for preeclampsia.

Purpose of the Study:

  • To test the hypothesis that preeclamptic patients exhibit proximal tubule epithelial injury.
  • To determine if lysosomal enzyme excretion patterns differ between preeclamptic and normotensive pregnant women.
  • To assess the potential of lysosomal enzymes as diagnostic/predictive markers for preeclampsia.

Main Methods:

  • Maternal serum and urine samples were collected from 14 preeclamptic and 28 normotensive pregnant women.

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  • Fluorometric assays were used to measure five specific lysosomal hydrolases: beta-glucuronidase, beta-hexosaminidase, alpha-galactosidase, beta-galactosidase, and alpha-mannosidase.
  • Statistical analysis (Mann-Whitney test) compared enzyme activities and fractional excretions between groups.

    • Main Results:

    • Serum activities of beta-hexosaminidase, alpha-galactosidase, and alpha-mannosidase were significantly lower in preeclamptic patients.
    • Urine activities of beta-glucuronidase, alpha-galactosidase, beta-galactosidase, and alpha-mannosidase were significantly higher in preeclamptic patients.
    • Fractional excretion of all five lysosomal hydrolases was significantly elevated in preeclamptic women compared to controls.

    Conclusions:

    • Preeclampsia is associated with decreased serum and increased urine activities of specific lysosomal hydrolases.
    • Elevated fractional excretion of all five studied lysosomal hydrolases in preeclampsia suggests kidney tubule dysfunction.
    • Lysosomal enzyme profiles in urine and serum show potential as diagnostic or predictive markers for preeclampsia.