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Cell-to-cell scanning in capillary electrophoresis
H A Fishman1, O Orwar, N L Allbritton
1Department of Chemistry, Stanford University, California 94305, USA.
Analytical Chemistry
|April 1, 1996
Summary
Cell-based biosensors overcome receptor desensitization using a scanning format. This method directs analytes to fresh cells, ensuring consistent responses for repetitive chemical analysis.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Cell Biology
Background:
- Cell-based biosensors face limitations due to receptor desensitization, hindering repetitive chemical analysis.
- Receptor desensitization leads to a loss of agonist-induced response, compromising sensor accuracy over time.
Purpose of the Study:
- To develop a novel cell-based biosensor strategy that overcomes receptor desensitization.
- To demonstrate response recovery in a scanning format for enhanced biosensor performance.
Main Methods:
- Utilized an array of immobilized NG108-15 cells for bradykinin B2 receptor studies.
- Employed capillary electrophoresis to fractionate analytes and direct them to a scanning array of cells.
- Implemented a scanning mechanism to expose cells to fresh agonists, preventing desensitization.
Main Results:
- Scanning to new cells fully restored the agonist response, overcoming desensitization observed with repetitive injections.
- Achieved a 100% success rate in detecting bradykinin in electrophoretic separations by pretesting and re-scanning cells.
- Demonstrated significant response recovery compared to traditional methods with marked reduction in cell response.
Conclusions:
- The scanning cell array format effectively overcomes receptor desensitization in cell-based biosensors.
- This approach enables reliable and repetitive chemical analysis by ensuring consistent cell responsiveness.
- The developed method offers a promising solution for improving the longevity and accuracy of biosensing applications.