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Related Experiment Videos

Fluorescent fiber-optic calcium sensor for physiological measurements

M Shortreed1, R Kopelman, M Kuhn

  • 1Department of Chemistry, University of Michigan, Ann Arbor 48109-1055, USA.

Analytical Chemistry
|April 15, 1996
PubMed
Summary

A novel optical sensor for calcium (Ca2+) was developed using a fluorescent indicator immobilized on an optical fiber. This sensor offers fast, accurate physiological measurements, even with high magnesium levels.

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Area of Science:

  • Biomedical Engineering
  • Chemical Sensors
  • Optical Sensing

Background:

  • Accurate monitoring of intracellular calcium (Ca2+) is crucial for understanding physiological processes.
  • Existing calcium sensors often face limitations in response time, dynamic range, or selectivity.
  • Developing robust optical sensors for real-time calcium measurements remains an active research area.

Purpose of the Study:

  • To construct and characterize a new optical sensor for calcium (Ca2+) detection.
  • To achieve a sensor with a dynamic range suitable for physiological measurements.
  • To evaluate the sensor's response time, lifetime, and selectivity in complex biological environments.

Main Methods:

  • Covalent immobilization of a novel calcium-selective, long-wavelength fluorescent indicator onto a silanized optical fiber.

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  • Utilizing photoinitiated copolymerization with acrylamide for indicator attachment.
  • Characterizing sensor performance, including response dynamics, lifetime, and selectivity against magnesium (Mg2+).

    • Main Results:

    • The developed optical sensor demonstrated a dynamic range optimal for physiological calcium (Ca2+) measurements.
    • Sensor response time was governed by aqueous diffusion, enabling monitoring of physiological rate fluctuations.
    • The sensor exhibited a high selectivity for calcium (Ca2+) over magnesium (Mg2+) with a calculated selectivity of 10(-4.5).
    • Sensor working lifetime was limited only by indicator photobleaching.

    Conclusions:

    • A new, robust optical sensor for calcium (Ca2+) has been successfully developed.
    • The sensor's fast response time and high selectivity make it suitable for real-time physiological monitoring.
    • This technology holds promise for advancing research in cellular signaling and related biomedical applications.