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Specimen collection and storage for diagnostic molecular pathology investigation
D H Farkas1, K L Kaul, D L Wiedbrauk
1Department of Clinical Pathology, William Beaumont Hospital, Royal Oak, MI 48073-6769, USA.
Abstract:
The success of the newest discipline in the diagnostic clinical pathology laboratory, molecular pathology, is dependent on proper collection and storage of both the original and processed (nucleic acid) specimen. This issue will grow in importance as test volumes increase in the diagnostic molecular pathology laboratory. This review is a distillation of a literature review by the Patient Preparation and Specimen Handling Committee of the College of American Pathologists. It describes specific collection, storage, and anticoagulant or preservative requirements based on the diagnostic molecular technique and/or specimen type used for analysis. This review serves as a guide for clinical laboratories interested in appropriate collection and storage of specimens to be used in nucleic acid-based analysis.
Insights
Proper specimen collection and storage are crucial for molecular pathology diagnostics. This guide details requirements for nucleic acid-based analysis to ensure accurate test results in clinical laboratories.
Area of Science:
- Clinical Pathology
- Molecular Diagnostics
- Laboratory Medicine
Background:
- Molecular pathology is a rapidly growing discipline within diagnostic clinical laboratories.
- Accurate diagnostic testing relies heavily on the integrity of collected specimens.
- Increasing test volumes necessitate standardized protocols for specimen handling.
Purpose of the Study:
- To provide a comprehensive guide on specimen collection and storage for molecular pathology.
- To outline specific requirements based on diagnostic techniques and specimen types.
- To ensure the quality and reliability of nucleic acid-based analyses in clinical settings.
Main Methods:
- Distillation of a literature review conducted by the College of American Pathologists' Patient Preparation and Specimen Handling Committee.
- Compilation of specific collection, storage, and anticoagulant/preservative requirements.
- Categorization of requirements by diagnostic molecular technique and specimen type.
Main Results:
- Detailed recommendations for specimen handling in molecular pathology.
- Identification of critical factors influencing nucleic acid integrity.
- Guidance on appropriate anticoagulants or preservatives for various analyses.
Conclusions:
- Adherence to proper specimen collection and storage protocols is essential for molecular pathology success.
- This review serves as a vital resource for clinical laboratories performing nucleic acid-based testing.
- Standardized practices will enhance the accuracy and efficiency of molecular diagnostic services.