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Related Experiment Videos

Kell typing by allele-specific PCR (ASP)

N D Avent1, P G Martin

  • 1International Blood Group Reference Laboratory, Southmead, Bristol.

British Journal of Haematology
|June 1, 1996
PubMed
Summary
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A new PCR test accurately determines Kell (K1) blood type, crucial for preventing hemolytic disease of the newborn in alloimmunized pregnancies. This method aids in prenatal Kell blood group typing.

Area of Science:

  • Immunology
  • Genetics
  • Transfusion Medicine

Background:

  • The Kell blood group system, particularly the Kell antigen (K1), is a significant immunogen in alloimmunized pregnancies, leading to hemolytic disease of the newborn.
  • The K/k polymorphism results from a point mutation in the Kell glycoprotein, altering Thr193 to Met193 and affecting N-glycan addition.

Purpose of the Study:

  • To develop and validate a simple Polymerase Chain Reaction (PCR) test for Kell (K1) blood group typing.
  • To assess the utility of this PCR test for prenatal determination of Kell type.

Main Methods:

  • Development of an allele-specific K-primer for PCR-based Kell blood group typing.
  • Validation of the PCR test using 65 genomic DNA samples from amniocytes and peripheral blood lymphocytes.

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Main Results:

  • The developed PCR test demonstrated complete concordance with serological Kell blood group typing.
  • The test accurately identified the K/k polymorphism based on the specific point mutation.

Conclusions:

  • A simple and accurate PCR test for Kell blood group typing has been established.
  • This PCR method is suitable for prenatal diagnosis of Kell type, aiding in the management of pregnancies at risk for hemolytic disease of the newborn.