Analysis of STR-PCR products with high-resolution denaturing PAGE

The Pharmacia Multiphor II horizontal electrophoresis chamber is a widespread tool for analysis of PCR products in forensic casework. Up to date, however, there is no protocol for successfully running high-resolution denaturing PAGE (poly-acrylamide gel electrophoresis) on a horizontal electrophoresis chamber. We modified the electrophoresis conditions to make this possible.