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Fatty acid transfer in taurodeoxycholate mixed micelles
1Department of Nutritional Sciences, Rutgers University, Cook College, New Brunswick, New Jersey 08903, USA.
Biochemistry
|June 11, 1996
Summary
Dietary lipid absorption is more efficient from micelles than vesicles. Fatty acid and monoacylglycerol transfer from vesicles may explain lipid uptake during bile salt insufficiency.
Area of Science:
- Biochemistry
- Gastroenterology
- Lipid Metabolism
Background:
- Dietary triacylglycerol digestion yields fatty acids and monoacylglycerol.
- These products are solubilized by bile into micellar and vesicular phases.
- Lipid absorption is thought to occur from both phases.
Purpose of the Study:
- To investigate the transfer of fatty acids and monoacylglycerol between micelles.
- To compare lipid transfer rates between micellar and vesicular phases.
- To assess the role of different phases in dietary lipid absorption.
Main Methods:
- A fluorescence self-quenching assay was used.
- Anthroyloxy-labeled lipids monitored transfer from donor to acceptor micelles.
- Model systems investigated intermicellar and intervesicular transfer rates.
Main Results:
- Fatty acid transfer mechanism involves diffusional and collisional processes.
- Diffusional transfer of fatty acids and monoacylglycerol was 30-fold faster from micelles than vesicles.
- Intermicellar and intervesicular transfer rates were 3-fold higher for fatty acids than monoacylglycerol.
Conclusions:
- Lipid uptake is likely more efficient from micellar phases.
- Vesicular lipid transfer may contribute to dietary lipid absorption during bile salt insufficiency.