Related Experiment Video
Updated: Aug 18, 2026

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
Published on: February 25, 2011
Antigenic determinants of Streptococcus pyogenes ribosomes
1Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Abstract:
An attempt has been made to identify the ribosomal proteins responsible for the production of antibodies upon immunization of monkeys with 70S ribosomes of Streptococcus pyogenes group A 29 M type. Identification was carried out by immunoblotting after isolation and separation of the proteins of 70S, 50S and 30S ribosomal particles on one- and two-dimensional gel electrophoresis. Three major proteins of S. pyogenes 70S ribosomes with clearly expressed antigenic properties were identified by Western blotting. One of these proteins belonged to the 50S subunit and the other two--to the 30S ribosomal subunit. We assume that these proteins represent those of group A streptococci. As a result the antibodies are produced first of all against these antigenic proteins in contrast to the others evolutionary conservative ribosomal proteins.
More Related Videos
Related Concept Videos
Ribosomes
Ribosomal RNA Synthesis
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomes
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Determinants of Bacterial Pathogenicity and Virulence
Streptococcal Pharyngitis
Inhibitors of Bacterial Protein Synthesis

