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Separation of B-3 monodesamidoinsulin from human insulin by high-performance liquid chromatography under alkaline
C Yomota1, Y Yoshii, T Takahata
1National Institute of Health Sciences, Osaka Branch, Japan.
Journal of Chromatography. A
|January 15, 1996
Abstract:
The separation of human insulin (HI) and related compounds such as A-21 monodesamido HI (A-21DHI) and B-3 monodesamido HI (B-3DHI) was investigated using reversed-phase HPLC and capillary zone electrophoresis (CZE). In the case of HPLC under the usual acidic conditions, whereas A-21DHI was separately eluted, B-3DHI was included in the HI peak. Then it was found that by applying a novel ODS column resistant to alkaline conditions, a good separation of B-3DHI from HI could be achieved using an alkaline eluent, and two peaks corresponding to B-3DHI were eluted in front of the HI peak.