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Updated: Jul 26, 2026
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Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
Published on: June 25, 2017
Glucose uptake in the chronically dysfunctional but viable myocardium
M Mäki1, M Luotolahti, P Nuutila
1Department of Clinical Physiology, University of Turku, Finland.
Circulation
|May 1, 1996
Summary
Insulin significantly increases glucose uptake in chronically dysfunctional but viable heart muscle in humans. This finding supports metabolic interventions for heart conditions with preserved insulin sensitivity.
Area of Science:
- Cardiology
- Nuclear Medicine
- Metabolic Research
Background:
- Human studies on glucose uptake regulation in dysfunctional but viable myocardium are lacking.
- Chronic myocardial dysfunction can result from occluded coronary arteries.
Purpose of the Study:
- To investigate glucose uptake regulation in chronically dysfunctional but viable human myocardium.
- To determine the effect of insulin on myocardial glucose uptake in these specific conditions.
Main Methods:
- Positron emission tomography (PET) with 2-fluoro-2-deoxy-D-glucose (FDG) was used to measure glucose uptake.
- Hyperinsulinemic euglycemic clamping assessed insulin's effect on glucose uptake.
- Myocardial blood flow was measured using oxygen-15-labeled water (H2O).
Main Results:
- Dysfunctional myocardium showed slightly reduced blood flow but increased glucose uptake in a fasting state.
- Insulin clamping induced a significant increase in glucose uptake in both dysfunctional and normal myocardial regions.
- Revascularization in patients confirmed tissue viability and subsequent wall-motion recovery.
Conclusions:
- Insulin-mediated glucose uptake is preserved in chronically dysfunctional but viable myocardium.
- These findings provide a basis for using metabolic interventions in patients with such myocardial conditions.
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