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Calcium transported to isolated rat liver nuclei by nicotinamide adenine dinucleotide is insensitive to thapsigargin
1Laboratoire de Neurobiologie Moléculaire des Interactions Cellulaires, Centre de Neurochimie du Centre National de la Recerche Scientifique, Strasbourg, France.
Abstract:
Calcium uptake by isolated nuclei was mediated by nicotinamide adenine dinucleotide. Oxidized nicotinamide nucleotide analogues were more effective mediators of nuclear calcium uptake. Thapsigargin inhibited ATP-mediated nuclear calcium transport without affecting NAD-mediated nuclear calcium uptake. Whilst DBHQ did not influence ATP-induced calcium transport, it did stimulate NAD-mediated nuclear calcium entry. Calcium channel blockers did not influence the action of NAD. This study provides a further mechanism for nuclear calcium transport regulated by changes in the cytosolic NAD(+)/NADH ratio.