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Complete nucleotide sequence of the mouse CTLA8 gene
Gene
|February 12, 1996
Summary
Researchers cloned the mouse cytotoxic T-lymphocyte-associated antigen 8 (CTLA8) gene, correcting a previous rat sequence misidentification. This mouse CTLA8 gene has two exons and one intron, with regulatory motifs identified.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- Cytotoxic T-lymphocyte-associated antigen 8 (CTLA8) plays a role in immune regulation.
- Previous studies reported a mouse CTLA8 sequence that is now identified as being of rat origin.
Purpose of the Study:
- To clone and determine the complete nucleotide sequence of the mouse CTLA8 gene.
- To characterize the structure and regulatory elements of the mouse CTLA8 gene.
- To compare the mouse CTLA8 sequence with related genes.
Main Methods:
- Gene cloning and nucleotide sequencing.
- Polymerase chain reaction (PCR) analysis.
- Bioinformatic analysis of gene structure and sequence homology.
Main Results:
- The complete nucleotide sequence of the mouse CTLA8 gene was determined.
- Sequence analysis confirmed the published CTLA8 sequence was of rat, not mouse, origin.
- The mouse CTLA8 gene consists of two exons and one intron, with identified transcription factor binding motifs and RNA instability motifs.
Conclusions:
- The full-length mouse CTLA8 protein comprises 147 amino acids.
- Mouse CTLA8 shares 88% amino acid identity with rat CTLA8 and 57% with HSV13 (an HVS ORF).
- Accurate characterization of mouse CTLA8 provides a foundation for further functional studies in immunology.