A cDNA encoding canine muscle-type phosphofructokinase
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Updated: Nov 5, 2026

Functional Site-Directed Fluorometry in Native Cells to Study Skeletal Muscle Excitability
Published on: June 2, 2023
B F Smith1, P S Henthorn, Y Rajpurohit
1School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104-6010, USA. smithbf@mail.auburn.edu
Abstract:
The canine muscle-type-phosphofructokinase-encoding gene (M-PFK) was sequenced by using a combination of cDNA cloning and RT-PCR amplification. The canine M-PFK sequence shares 88 and 90% identity with rabbit and human M-PFK, respectively. The canine ORF was determined to be 6-bp longer than either human or rabbit M-PFK due to a 6-bp insertion at the end of exon 13.
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