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The LYS5 gene of Saccharomyces cerevisiae
K G Miller1, J K Bhattacharjee
1Department of Microbiology, Miami University, Oxford, OH 45056, USA.
Abstract:
The LYS2 and LYS5 genes of Saccharomyces cerevisiae together encode the 180-kDa alpha-aminoadipate reductase (AAR) in the biosynthetic pathway of lysine. The 4.8-kb LYS2 gene encodes the 155-kDa subunit of AAR. The complete nucleotide (nt) sequence of the 1.1-kb LYS5 gene is presented in this report. It contains a single continuous open reading frame of 816 nt encoding a 272-amino-acid, 30.6-kDa polypeptide.
Insights
The LYS2 and LYS5 genes in yeast encode alpha-aminoadipate reductase (AAR), crucial for lysine biosynthesis. This study details the LYS5 gene sequence and its encoded polypeptide.
Area of Science:
- Biochemistry
- Molecular Biology
- Yeast Genetics
Background:
- The LYS2 and LYS5 genes in Saccharomyces cerevisiae are essential for lysine biosynthesis.
- These genes collectively encode the alpha-aminoadipate reductase (AAR) enzyme, a key component in the lysine biosynthetic pathway.
- The LYS2 gene encodes a 155-kDa subunit of AAR.
Purpose of the Study:
- To present the complete nucleotide sequence of the LYS5 gene.
- To characterize the polypeptide encoded by the LYS5 gene.
Main Methods:
- Nucleotide sequencing of the LYS5 gene.
- Bioinformatic analysis to identify open reading frames and predict protein characteristics.
Main Results:
- The complete nucleotide sequence of the 1.1-kb LYS5 gene was determined.
- A single continuous open reading frame of 816 nucleotides was identified within LYS5.
- This open reading frame encodes a 272-amino-acid polypeptide with a molecular mass of 30.6-kDa.
Conclusions:
- The LYS5 gene encodes a specific subunit of the alpha-aminoadipate reductase enzyme.
- The detailed sequence information provides a foundation for further functional and structural studies of AAR in yeast.