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Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae
J O Mascorro-Gallardo1, A A Covarrubias, R Gaxiola
1Departamento de Biología Molecular de Plantas, UNAM, Cuernavaca, Morelos, Mexico.
Gene
|June 12, 1996
Abstract:
We report the construction and characterization of a yeast shuttle-expression vector that can be used to express genes in yeast in a modulated form. This vector is centromeric, with a polylinker that optionally can provide an ATG start of translation. Expression features are based on the CUP1 yeast metallothionein gene promoter, which can be tightly modulated by copper.