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Novel specific endonuclease activity recognizing a 10-bp sequence
1Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA. LEROTC@biomed.med.yale.edu
Gene
|June 12, 1996
Abstract:
We report here the generation of a novel restriction endonuclease (ENase) activity with the 10-bp recognition sequence, [symbol: see text]. This specificity could be achieved by first methylating a substrate DNA with M.MamI in vivo, followed by in vitro R.DpnI restriction.