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Related Experiment Videos

Ultrastructural observations on the peritoneum in the mouse

R R Ettarh1, K E Carr

  • 1Department of Anatomy, University College of Dublin, Ireland, UK.

Journal of Anatomy
|February 1, 1996
PubMed
Summary

This study reveals a novel surface layer on mouse small intestine serosa, potentially preserving peritoneal fluid. Mesenteric stomata were observed, possibly explaining differences in parietal peritoneum and mesentery permeability.

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Area of Science:

  • Gastroenterology
  • Cell Biology
  • Microscopy

Background:

  • The serous mesothelium lining the abdominal cavity plays a crucial role in peritoneal function.
  • Understanding the ultrastructure of the mesothelium is key to comprehending peritoneal fluid dynamics and transport.

Purpose of the Study:

  • To investigate the ultrastructural characteristics of the mouse small intestine serosa and mesentery using advanced microscopy techniques.
  • To identify and characterize any novel surface features or structures present on the mesothelium.

Main Methods:

  • Scanning electron microscopy (SEM) for surface visualization.
  • Transmission electron microscopy (TEM) for detailed ultrastructural analysis.
  • Examination of mouse small intestine serosa and mesentery.

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Main Results:

  • Identified a unique amorphous surface layer on parts of the serosa, likely trapped serous fluid, resistant to initial cleaning but lost during TEM processing.
  • Observed stomata in the mesentery, with no discernible connection to the lymphatic system.
  • Characterized the serosa as a single layer of flattened, microvilli-bearing cells with specific organelles.

Conclusions:

  • The novel surface layer may offer a method for preserving and assessing peritoneal fluid in future studies.
  • The presence of mesenteric stomata could account for observed differences in permeability between the parietal peritoneum and mesentery.