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Herpes virus-like sequences are specifically found in Kaposi sarcoma lesions
E O'Neill1, T H Henson, A J Ghorbani
1Department of Virology/Molecular Biology, St Jude Children's Research Hospital, Memphis, TN 38105, USA.
Aim:
To detect the prevalence of herpes virus-like DNA sequences in AIDS associated Kaposi sarcoma (KSHV) lesions and normal tissue.
Methods:
KSHV detection was performed by polymerase chain reaction (PCR) using four different sets of primers. PCR products were cloned, sequenced, and analysed.
Results:
All of four biopsies of Kaposi sarcoma lesions and all of three paraffin embedded Kaposi sarcoma tissues were positive for KSHV, while normal tissue from the same patients was negative. Sequence analysis of amplification products revealed polymorphisms that result in amino acid changes of the predicted sequence.
Conclusions:
KSHV is prevalent in tissues from Kaposi sarcoma, suggesting a role in the development of the tumour. On this basis, anti-herpes virus agents should be considered to control Kaposi sarcoma.