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Related Experiment Videos

Optic nerve-dependent changes in adult frog tectal cell phenotypes

Q Liu1, E A Debski

  • 1School of Biological Sciences, University of Kentucky, Lexington 40506, USA.

Journal of Neurobiology
|April 1, 1996
PubMed
Summary

The optic nerve regulates substance P and serotonin levels in frog optic tectum. Optic nerve transection altered the expression of these neurotransmitters in the deafferented and afferented tectal lobes.

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Area of Science:

  • Neuroscience
  • Neurobiology
  • Cellular Neuroscience

Background:

  • Optic nerve activity influences retinal ganglion cell terminal placement in the frog optic tectum.
  • The role of the optic nerve in regulating neurotransmitter biosynthesis within its target structures remains largely unexplored.

Purpose of the Study:

  • To investigate whether the optic nerve influences neurotransmitter biosynthesis, specifically substance P and serotonin, in the optic tectum.
  • To determine if optic nerve presence affects the percentage and intensity of substance P-like immunoreactive (SP-ir) and serotonin-like immunoreactive (5-HT-ir) cells.

Main Methods:

  • Unilateral optic nerve transections were performed on adult frogs, preventing regeneration.
  • Percentage and intensity of SP-ir and 5-HT-ir cells were assayed in deafferented and afferented tectal lobes at 6 weeks and 5 months post-lesion.

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  • Comparisons were made between lesioned and normal animals.

    • Main Results:

    • The percentage of SP-ir cells was significantly lower in afferented tectal lobes compared to deafferented ones.
    • A significant increase in the percentage of 5-HT-ir cells was observed in the deafferented tectum.
    • The intensity of 5-HT-ir cells was significantly greater in the deafferented lobe, while SP-ir cell intensity showed only a transient decrease.

    Conclusions:

    • The optic nerve actively regulates substance P and serotonin expression in the optic tectum.
    • The regulation of substance P and serotonin by the optic nerve likely involves distinct molecular pathways.
    • These findings highlight the complex interplay between neural activity and neurotransmitter homeostasis in the visual system.