Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Defects in enteric innervation and kidney development in mice lacking GDNF

J G Pichel1, L Shen, H Z Sheng

  • 1Laboratory of Mammalian Genes and Development, National Institutes of Health, Bethesda 20892, USA.

Nature
|July 4, 1996
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An observational study of farmer-reported clinical mastitis in New Zealand dairy ewes.

New Zealand veterinary journal·2024
Same author

Regional binding of tau and amyloid PET tracers in Down syndrome autopsy brain tissue.

Molecular neurodegeneration·2020
Same author

Volumetric MRI Study of the Brain in Fetuses with Intrauterine Cytomegalovirus Infection and Its Correlation to Neurodevelopmental Outcome.

AJNR. American journal of neuroradiology·2019
Same author

Molecular detection of Bartonella coopersplainsensis and B. henselae in rats from New Zealand.

New Zealand veterinary journal·2018
Same author

The Effect of Urine Concentration and pH on the Growth of Escherichia Coli in Canine Urine In Vitro.

Journal of veterinary internal medicine·2018
Same author

The Noradrenergic System of Aged GDNF Heterozygous Mice.

Cell transplantation·2017

Glial cell line-derived neurotrophic factor (GDNF) is crucial for kidney development and enteric nervous system formation. GDNF null mutant mice exhibit kidney agenesis and impaired gastrointestinal innervation, highlighting its essential developmental roles.

Area of Science:

  • Developmental Biology
  • Neuroscience
  • Genetics

Background:

  • Glial cell line-derived neurotrophic factor (GDNF) is a neurotrophic factor supporting midbrain dopaminergic neurons.
  • GDNF exhibits broad neurotrophic activity in vitro and in vivo, indicating therapeutic potential for neurological disorders.
  • GDNF expression during mammalian development extends beyond the nervous system to the kidney and gastrointestinal tract, suggesting roles in organogenesis.

Purpose of the Study:

  • To investigate the role of GDNF in mammalian development.
  • To determine the necessity of GDNF for kidney and enteric nervous system development.

Main Methods:

  • Generation of a null mutation in the murine GDNF locus.
  • Phenotypic analysis of GDNF-deficient mice, focusing on kidney and enteric innervation.

Related Experiment Videos

Main Results:

  • GDNF null mutant mice displayed kidney agenesis or dysgenesis.
  • Mutant mice showed defective enteric innervation of the gastrointestinal tract.
  • GDNF was demonstrated to induce ureter bud formation and branching during metanephros development.

Conclusions:

  • GDNF is essential for proper kidney development, including ureteric bud induction and branching.
  • GDNF plays a critical role in the development and innervation of the gastrointestinal tract.
  • These findings underscore GDNF's significance in mammalian organogenesis and neural development.