Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A quasi-monoclonal mouse

M Cascalho1, A Ma, S Lee

  • 1Department of Microbiology and Immunology, University of California, San Francisco 94143-0670, USA.

Science (New York, N.Y.)
|June 14, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Effectiveness of early proactive telephone follow-up on perioperative medication adherence in patients with atrial fibrillation].

Zhonghua nei ke za zhi·2026
Same author

Night shifts and sleep among Chinese nursing students on internship.

Occupational medicine (Oxford, England)·2025
Same author

[Expression of GPRC5D in newly diagnosed patients with multiple myeloma detected by flow cytometry and its prognostic value].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2025
Same author

The Multi-domain Lifestyle Intervention for Cognitive Impairment in Community-Dwelling Older Adults in Hangzhou (The Heritage Study): Study Design and Protocol.

The journal of prevention of Alzheimer's disease·2024
Same author

CT-based radiomics for prediction of pulmonary haemorrhage after percutaneous CT-guided transthoracic lung biopsy of pulmonary nodules.

Clinical radiology·2023
Same author

[Prediction of 1p/19q codeletion status in diffuse lower-grade glioma using multimodal MRI radiomics].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University·2023
Same journal

A native sulfur deposit in Gale crater, Mars.

Science (New York, N.Y.)·2026
Same journal

Coordinated demise of harmful algal blooms.

Science (New York, N.Y.)·2026
Same journal

Genetic effects put into context.

Science (New York, N.Y.)·2026
Same journal

Bacteria share proteins to survive antibiotics.

Science (New York, N.Y.)·2026
Same journal

Impacts shaped Earth's first continents.

Science (New York, N.Y.)·2026
Same journal

Erratum for the Report "Covalently bonded single-molecule junctions with stable and reversible photoswitched conductivity" by C. Jia <i>et al</i>.

Science (New York, N.Y.)·2026
See all related articles

This study created a quasi-monoclonal mouse to investigate antibody diversity. Results show that while initial antibody specificity is limited, mutations alter antigen receptors, but don't fully explain serum immunoglobulin levels.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Antibody diversity is crucial for adaptive immunity.
  • Understanding the generation of antibody diversity requires precise models.
  • Previous models did not fully capture the complexity of antibody repertoire generation.

Purpose of the Study:

  • To generate a gene-targeted mouse model for studying antibody diversity.
  • To investigate the role of somatic hypermutation and secondary rearrangements in shaping the antibody repertoire.
  • To determine factors influencing serum immunoglobulin concentrations.

Main Methods:

  • Gene targeting to create a mouse hemizygous for a rearranged immunoglobulin (Ig) heavy chain V(D)J segment.
  • Generation of a mouse lacking functional kappa light chain alleles.

Related Experiment Videos

  • Characterization of antibody specificity using the hapten (4-hydroxy-3-nitrophenyl) acetyl (NP).
  • Analysis of B cell antigen receptors and serum immunoglobulin isotypes.

    • Main Results:

    • The quasi-monoclonal mouse produced a heavy chain specific for NP when paired with lambda light chains.
    • The primary antibody repertoire was monospecific, but 20% of antigen receptors changed specificity due to somatic hypermutation and secondary rearrangements.
    • Serum immunoglobulin concentrations were similar to nontransgenic littermates, but only a fraction of IgM and no other isotypes bound NP.

    Conclusions:

    • Somatic hypermutation and secondary rearrangements significantly alter antibody specificity in this model.
    • Neither network interactions nor random B cell activation fully explain observed serum immunoglobulin concentrations.
    • This quasi-monoclonal mouse model provides insights into the mechanisms of antibody repertoire generation and regulation.