[Cloning leishmania at the promastigote stage using the Vonbrun micromanipulator]

A method was worked out for cloning Leishmania strains at the promastigote stage from cultures reared on two-phase nutrient medium by means of Vonbrün micromanipulator. By this method cultures of Leishmania can be obtained from one cell of these protozoans. During the cloning of Leishmania strains isolated from experimentally and naturally infected Phlebotomus papatasi and Ph. caucasicus the per cent of obtaining the clones from isolated promastigotes cultivated on a nutrient medium depends on the absolute and relative age of culture and on the growth intensity of the strain on a nutrient medium.