Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nucleic acids02:43

Nucleic acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Nucleic Acids02:43

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An examination of mindfulness on Mu suppression and pain empathy and its relation to trait empathy.

Social neuroscience·2023
Same author

Harmonizing Definitions for Diagnostic Criteria and Prognostic Assessment of Transplantation-Associated Thrombotic Microangiopathy: A Report on Behalf of the European Society for Blood and Marrow Transplantation, American Society for Transplantation and Cellular Therapy, Asia-Pacific Blood and Marrow Transplantation Group, and Center for International Blood and Marrow Transplant Research.

Transplantation and cellular therapy·2022
Same author

In reply to Huo et al.: Treating small hepatocellular carcinoma: Stereotactic body radiation therapy versus radiofrequency ablation.

Journal of gastroenterology and hepatology·2020
Same author

Dosimetric analysis of stereotactic rotational versus static intensity-modulated radiation therapy for pancreatic cancer.

Cancer radiotherapie : journal de la Societe francaise de radiotherapie oncologique·2018
Same author

Abdominal adiposity intensifies the negative effects of ambient air pollution on lung function in Korean men.

International journal of obesity (2005)·2017
Same author

Generation of RUNX3 knockout pigs using CRISPR/Cas9-mediated gene targeting.

Reproduction in domestic animals = Zuchthygiene·2016
Same journal

Wired for Corruption: Inter-Brain Synchrony Encodes Bribery-Related Value Information and Predicts Bribery Agreement.

Annals of the New York Academy of Sciences·2026
Same journal

LM-YOLO: A Lightweight Multi-Scale Enhanced Model for Forest Smoke Detection Using Unmanned Aerial Vehicles.

Annals of the New York Academy of Sciences·2026
Same journal

Polyrhythm Perception and Production: A Scoping Review.

Annals of the New York Academy of Sciences·2026
Same journal

DARTS-CNN-BiLSTM: Intelligent Fault Diagnosis for Computer Numerical Control Machine Tool Feed System.

Annals of the New York Academy of Sciences·2026
Same journal

Synchrony and Reciprocity in Rhythmic Interaction.

Annals of the New York Academy of Sciences·2026
Same journal

Leveraging Salt Fortification Platforms to Address Multiple-Micronutrient Deficiencies in Africa: A Policy Opportunity.

Annals of the New York Academy of Sciences·2026
See all related articles

Related Experiment Video

Updated: Jul 1, 2026

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins
12:24

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

Published on: July 3, 2010

RNA libraries and RNA recognition

F J Schmidt1, B Cho, H B Nicholas

  • 1Department of Biochemistry, University of Missouri-Columbia 65203, USA.

Annals of the New York Academy of Sciences
|May 15, 1996
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method to isolate RNA aptamers that bind other nucleic acids without relying on Watson-Crick base pairing. This technique identified novel RNA-RNA interaction motifs, potentially useful for therapeutic applications.

More Related Videos

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

Related Experiment Videos

Last Updated: Jul 1, 2026

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins
12:24

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

Published on: July 3, 2010

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Aptamers are nucleic acid molecules selected for specific ligand binding.
  • Previous RNA aptamer selections for nucleic acid ligands primarily yielded Watson-Crick binding molecules.
  • Non-Watson-Crick RNA-RNA interactions are known in other biological contexts.

Purpose of the Study:

  • To develop a selection strategy for isolating RNA aptamers that bind nucleic acids via non-Watson-Crick interactions.
  • To identify novel RNA-RNA interaction motifs beyond standard base pairing.

Main Methods:

  • Construction of large random RNA libraries (> 10^13 sequences).
  • Incorporation of a selection strategy to prevent Watson-Crick base pairing during selection.
  • Affinity purification and amplification of RNA binders.
  • Characterization of isolated aptamers for binding specificity and sequence motifs.

Main Results:

  • Successfully isolated RNA aptamers that bind nucleic acid ligands without extensive Watson-Crick complementarity.
  • The selection strategy effectively obviated Watson-Crick interactions.
  • Identified a recurring sequence motif (oligo-G stretches bounded by U residues) in isolated aptamers.
  • This motif may represent a general RNA-RNA interaction motif, similar to telomeric DNA.

Conclusions:

  • A novel selection strategy can isolate RNA aptamers recognizing nucleic acids through non-Watson-Crick interactions.
  • The identified sequence motif (oligo-G/U) is a potential key element in RNA-RNA recognition.
  • This work expands the understanding of RNA-RNA interactions and aptamer selection methodologies.