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Construction of gene expression system in cultured tobacco cells
A Shinmyo1, K Yoshida, T Kasai
1Graduate School of Biological Science, Nara Institute of Science and Technology, Japan.
Annals of the New York Academy of Sciences
|May 15, 1996
Abstract:
To construct a gene expression system in cultured tobacco cells, useful regulatory elements of plant genes were studied. The promoter of the horseradish peroxidase gene, prxC2, showed high activity in tobacco cells, and it contained enhancer sequences and a cis element for wound induction. The heat shock promoter of the HSP18.2 gene from A. thaliana had strong activity of transcription when the incubation temperature of tobacco cells was shifted from 25 degrees C to 37 degrees C. These elements could be good candidates for foreign gene expression in tobacco cells.