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Characterization of Saccharomyces cerevisiae deficient in expression of phospholipase D
1Department of Cell and Molecular Pharmacology, Medical University of South Carolina, Charleston, 29425-2251, USA.
The Biochemical Journal
|February 15, 1996
Abstract:
A gene encoding phospholipase D (PLD) in Saccharomyces cerevisiae was identified. The 195 kDa product of PLD1 has 24% overall sequence identity with a plant PLD. Expression of yeast PLD activity was eliminated by one-step gene disruption. Yeast haploids lacking PLD activity were deficient in growth on non-fermentable carbon sources. Diploids lacking expression of PLD1 were unable to sporulate.