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cDNA cloning of a novel CYP3A from rat brain
H Wang1, H Kawashima, H W Strobel
1Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston, 77225, USA.
Abstract:
One full length cDNA clone, designated 3aH15, was isolated from a control male rat brain cDNA library. 3aH15 encoded a protein composed of 503 amino acid residues. The deduced amino acid sequence of 3aH15 was 92% identical to Cyp3a-13 and had a 68.4% to 76.5% homology with the other reported CYP3A sequences. Clone 3aH15 was thus named CYP3A9. No significant induction of the CYP3A9 expression in rat brain by dexamethasone was observed by Northern blot analysis. CYP3A9 cDNA was expressed in E. coli and the expressed P450 3A9 is active in the demethylation of erythromycin as well as benzphetamine.