Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cloning and expression of a rat brain basic helix-loop-helix factor

H Kawakami1, H Maruyama, M Yasunami

  • 1Third Department of Internal Medicine, Hiroshima University School of Medicine, Japan.

Biochemical and Biophysical Research Communications
|April 5, 1996
PubMed
Summary

Researchers identified brain basic helix-loop-helix factor 1 (BHF1) in rats, crucial for neuron development. BHF1 shows dominant expression in the cerebellum, suggesting a role in its specific functions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hepatocyte growth factor is linked by O-glycosylated oligosaccharide on the alpha chain.

Biochemical and biophysical research communications·1992
Same author

Midline malignant B-cell lymphoma with leukemic transformation.

Cancer·1992
Same author

QRS complex changes in the V5 ECG lead during cardiac surgery.

Journal of cardiothoracic and vascular anesthesia·1992
Same author

[The successful treatment of G-CSF in autoimmune neutropenia with liver cirrhosis complicated by esophageal varices].

[Rinsho ketsueki] The Japanese journal of clinical hematology·1992
Same author

5-S-cysteinyldopa in urine and tumors.

The Journal of dermatology·1992
Same author

Effects of hyperthermia on the effectiveness of MK-801 treatment in the gerbil hippocampus following transient forebrain ischemia.

Brain research bulletin·1992

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Basic helix-loop-helix (BHLH) transcription factors regulate cell differentiation.
  • The BHLH family includes important regulators of neuronal development.
  • Brain-specific BHLH factors are critical for central nervous system development and function.

Purpose of the Study:

  • To clone and characterize novel rat brain-specific BHLH factors.
  • To investigate the expression patterns of these factors in different species and developmental stages.
  • To elucidate the potential roles of BHF1 in neuronal development and cerebellar function.

Main Methods:

  • Cloning of rat cDNAs for brain basic helix-loop-helix factor 1 (BHF1).
  • Sequence analysis and comparison with known BHLH proteins (e.g., MATH-2/NEX1).

Related Experiment Videos

  • mRNA expression analysis using techniques like Northern blotting or RT-PCR in adult bovine, rat, mouse, and mouse embryos.

    • Main Results:

    • Two rat cDNAs for BHF1 were successfully cloned, encoding a 357-amino acid protein with high identity to MATH-2/NEX1 in the BHLH domain.
    • BHF1 mRNA was predominantly expressed in the brain, with highest levels in the cerebellum, across adult bovine, rat, and mouse.
    • Distinct shorter BHF1 mRNA transcripts (1.6 kb and 1.8 kb) were observed in mouse embryos, decreasing with development.

    Conclusions:

    • BHF1 is a novel brain-specific BHLH factor with conserved expression across species.
    • The prominent cerebellar expression suggests a role for BHF1 in cerebellum-specific functions.
    • BHF1 may be involved in the developmental processes of neurons, particularly within the cerebellum.