Related Experiment Video
Updated: Jul 31, 2026

A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors
Published on: March 15, 2016
Endothelin stimulates sis-inducing factor-like DNA binding activity in CHO-K1 cells expressing ETA receptors
T C Peeler1, K M Conrad, K M Baker
1Weis Center for Research, Geisinger Clinic, Danville, Pennsylvania 17822, USA.
Abstract:
ET-1, a member of the family of peptides known as endothelins, binds to a G-protein-coupled receptor, ET(A), and stimulates a variety of cellular responses, including contraction, growth, and mitogenesis. ET-1 stimulation of a chinese hamster ovary cell line stably transfected with the ET(A) receptor (CHO/ET(A)) induced formation of SIF (sis-inducing factor), a key component of the STAT (Signal Transducers and Activators of Transcription) pathway, in a concentration-dependent manner. SIF induction was blocked by a specific inhibitor of ET(A), BQ610, and by genistein, a tyrosine kinase inhibitor. This report demonstrates that ET-1 stimulates the STAT pathway of signal transduction through a G-protein-coupled receptor, ET(A), in this stably transfected cell line.
More Related Videos
09:20The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress
Published on: October 31, 2016
07:13Characterizing Modulators of Protease-Activated Receptors with a Calcium Mobilization Assay Using a Plate Reader
Published on: May 24, 2024
Related Concept Videos
Internal Receptors
Regulation of Angiogenesis and Blood Supply
Amplifying Signals via Enzymatic Cascade
The JAK-STAT Signaling Pathway
Intracellular Signaling Affects Focal Adhesions
Some...
Transducer Mechanism: Enzyme-Linked Receptors
Major types that are helpful drug targets include: