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An upstream positive regulatory element in human GM-CSF promoter is recognized by NF-kappa B/Rel family members
M Musso1, P Ghiorzo, P Fiorentini
1IBiG, Institute of Biology and Genetics, University of Genova, Italy.
Abstract:
To further extend the previous analysis of cis-acting elements and cognate trans-acting factors that contribute to GM-CSF transcriptional regulations, we have examined a promoter region between -1742 and -2010. DNase I footprinting assays showed four protected sequences named A, B, C and D. DNA transfections in the T-lymphoid Mo cell line, which constitutively expresses GM-CSF, indicated that the A element, located between -2002 and -1984, has a positive role on transcription. Further characterization by electrophoretic mobility shift assays in the presence of different competitor oligonucleotides showed that this element binds a factor of the NF-kappa B/Rel family.