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5-Nitrosothio-2-nitrobenzoate: a reagent for the nitrosation of thiol groups in proteins
M S Studebaker1, H Zhang, G E Means
1Department of Biochemistry, The Ohio State University, 484 West 12th Avenue, Columbus, Ohio, 43210, USA.
Analytical Biochemistry
|June 1, 1996
Abstract:
The S-nitroso derivative of 5-thio-2-nitrobenzoate was synthesized from 5,5'-dithiobis(2-nitrobenzoic acid) and partially characterized. Although relatively unstable, it is easy to prepare and reacts very rapidly with thiols and thiol groups of proteins to give corresponding S-nitrosothiols and 5-thio-2-nitrobenzoate dianion. The latter's easy spectrophotometric detection makes such reactions easy to follow and to quantitate.