Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Retinoids as Ig Isotype-Switch Modulators

Tokuyama1, Tokuyama

  • 1Department of Nuclear Medicine, Faculty of Medicine, Kanazawa University, Takaramachi, Kanazawa, Ishikawa-ken, 920, Japan

Cellular Immunology
|June 15, 1996
PubMed
Summary

Retinoids, including all-trans retinoic acid (RA), influence B-cell antibody isotype switching. RA and cytokines like IL-4 and IL-5 are critical for directing IgA and IgE production.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Slow dynamics of equilibrium density fluctuations in suspensions of colloidal hard spheres near the glass transition

Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics·2000
Same author

Enantioselective Total Synthesis of (+)-Gelsemine: Determination of Its Absolute Configuration This work was supported in part by the Ministry of Education, Sports, and Culture, Japan. S.Y. thanks the JSPS for a predoctoral fellowship.

Angewandte Chemie (International ed. in English)·2000
Same author

Both insulin sensitivity and glucose sensitivity are impaired in patients with non-diabetic liver cirrhosis.

Hepatology research : the official journal of the Japan Society of Hepatology·2000
Same author

Occurrence and significance of decahydroquinolines from dendrobatid poison frogs and a myrmicine ant: use of 1H and 13C NMR in their conformational analysis

Journal of natural products·1999
Same author

Nonequilibrium effects on slow dynamics in concentrated colloidal suspensions.

Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics·1996
Same author

Dynamics of hard-sphere suspensions.

Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics·1994

Area of Science:

  • Immunology
  • Molecular Biology

Background:

  • B-cell activation and antibody isotype switching are critical for adaptive immunity.
  • Cytokines like Interleukin-4 (IL-4) and Interleukin-5 (IL-5) play key roles in directing B-cell differentiation.
  • Retinoids, vitamin A derivatives, are known to have immunomodulatory effects.

Purpose of the Study:

  • To investigate the role of retinoids in directing B-cell isotype switching to IgA and IgG1 (IgE).
  • To analyze the combined effects of all-trans retinoic acid (RA) and Th2-type cytokines (IL-4, IL-5) on B-cell responses.

Main Methods:

  • LPS-stimulated murine B cells were used in vitro.
  • The production of IgA, IgG1, and IgE was measured under varying concentrations of RA, IL-4, and IL-5.

Main Results:

  • All-trans retinoic acid (RA) enhanced IgA production at high concentrations (10-100 nM) with IL-5.
  • IL-4 modulated IgA responses in a dose-dependent manner, inhibiting at high concentrations (>250 U/mL) and enhancing at low concentrations (<130 U/mL).
  • IL-4 alone strongly enhanced IgG1 and IgE responses, while IL-5 showed synergistic effects that could be attenuated by low RA concentrations.

Conclusions:

  • The concentration ratios of IL-4 and IL-5, along with retinoid concentrations, are crucial for initiating and directing B-cell isotype switching.
  • Retinoids can modulate, and potentially direct, specific antibody isotype production in response to cytokine signals.

Related Experiment Videos