Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapid detection of mitochondrial sequence polymorphisms using multiplex solid-phase fluorescent minisequencing

G Tully1, K M Sullivan, P Nixon

  • 1Service Development, The Forensic Science Service, Priory House, Birmingham, B5 6QQ, United Kingdom.

Genomics
|May 15, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reversing immunosuppression in the tumor microenvironment of fibrolamellar carcinoma via PD-1 and IL-10 blockade.

Scientific reports·2024
Same author

Pre-Transplant Marital Status and Hematopoietic Cell Transplantation Outcomes

Current oncology (Toronto, Ont.)·2020
Same author

High-dose intravenous immunoglobulin as adjuvant treatment for grade IV acute cutaneous graft-versus-host disease.

The British journal of dermatology·2019
Same author

Hypoxia as a barrier to immunotherapy in pancreatic adenocarcinoma.

Clinical and translational medicine·2019
Same author

High-dose BCNU/Melphalan conditioning regimen before autologous stem cell transplantation in newly diagnosed multiple myeloma.

Bone marrow transplantation·2017
Same author

Fructosamine; is the current interest in alternative glycaemic markers justified?

Diabetic medicine : a journal of the British Diabetic Association·2015
Same journal

Integrating transcriptomics and metabolomics reveals the molecular landscape of sperm maturation driven by regional differentiation in the epididymis of Guizhou-Guiqian semi-fine wool sheep.

Genomics·2026
Same journal

Impact of genotype on histopathology and clinical characters in a Chinese cohort with obstructive hypertrophic cardiomyopathy.

Genomics·2026
Same journal

A novel reusable transcriptome-wide association study workflow used to map key genes linked to important cattle traits.

Genomics·2026
Same journal

The large mitochondrial genome of Syndiclis anlungensis (Lauraceae): Genome structure, comparative analysis, and phylogenetic relationships with other Syndiclis species.

Genomics·2026
Same journal

DeepGEP: Deep learning for gene expression prediction from multi-omics in mammals.

Genomics·2026
Same journal

Molecular features of external Auditory Canal cholesteatoma by microbial metagenomic sequencing.

Genomics·2026
See all related articles

This study introduces multiplex solid-phase fluorescent minisequencing for detecting DNA mutations. This method aids in individual identification using mitochondrial DNA polymorphisms, with calculated match probabilities for different ethnic groups.

Area of Science:

  • Molecular Biology
  • Forensic Genetics
  • Biotechnology

Background:

  • Accurate individual identification is crucial in forensic science and population genetics.
  • Mitochondrial DNA (mtDNA) polymorphisms are valuable markers for human identification due to their high copy number and maternal inheritance.
  • Existing methods for analyzing mtDNA polymorphisms can be time-consuming and may not be suitable for simultaneous detection of multiple markers.

Purpose of the Study:

  • To develop and validate a novel method for simultaneous detection of multiple point mutations and small insertions/deletions in DNA.
  • To apply this method for the analysis of mitochondrial DNA polymorphisms for individual identification.
  • To establish a preliminary mtDNA database for British Caucasian and Afro-Caribbean populations to assess the method's utility in forensic applications.

Related Experiment Videos

Main Methods:

  • Multiplex solid-phase fluorescent minisequencing was employed for simultaneous genotyping of multiple mtDNA polymorphic sites.
  • DNA samples were collected from 152 British Caucasians and 103 British Afro-Caribbeans.
  • A database of mtDNA polymorphisms was constructed, and statistical analysis was performed to calculate the probability of a random match.

Main Results:

  • The multiplex solid-phase fluorescent minisequencing method successfully detected multiple point mutations and small insertions/deletions simultaneously.
  • The analysis of mtDNA polymorphisms yielded distinct profiles for individuals within the studied populations.
  • The calculated probability of a chance match between two unrelated individuals was 0.054 for Caucasians and 0.026 for Afro-Caribbeans.

Conclusions:

  • Multiplex solid-phase fluorescent minisequencing is an effective and efficient method for simultaneous analysis of multiple DNA polymorphisms.
  • The method is suitable for individual identification using mitochondrial DNA, offering a powerful tool for forensic genetics.
  • The established mtDNA database provides valuable data for forensic casework involving British Caucasian and Afro-Caribbean individuals.