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The human PECAM1 gene maps to 17q23
R J Gumina1, N E Kirschbaum, P N Rao
1Department of Cellular Biology and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin.
Genomics
|June 1, 1996
Summary
The gene for Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1) has been precisely mapped to chromosome 17q23. This finding aids in understanding the genetic basis of cell adhesion and related molecular functions.
Area of Science:
- Genetics
- Molecular Biology
- Cell Biology
Background:
- Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1) is a key molecule in cell adhesion.
- Several adhesion molecule genes are located on chromosome 17, suggesting potential functional or evolutionary links.
Purpose of the Study:
- To determine the precise chromosomal and regional location of the PECAM1 gene.
- To investigate potential colocalization of PECAM1 with other adhesion molecule genes on chromosome 17.
Main Methods:
- Polymerase chain reaction (PCR)-based analysis of somatic cell hybrids.
- Analysis of a regional mapping panel for human chromosome 17.
- Fluorescence in situ hybridization (FISH).
Main Results:
- The PECAM1 gene was localized to chromosome 17.
- Further analysis refined the location to the long arm, specifically the 17q23-qter region.
- FISH confirmed the PECAM1 locus assignment to the 17q23 region.
Conclusions:
- The precise location of the PECAM1 gene is 17q23.
- This localization provides a basis for further studies into the regulation and function of PECAM-1 in relation to other chromosome 17-linked adhesion molecules.