Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

5' Terminal noncoding sequence heterogeneity in reovirus mRNA

K E Hastings, S Millward

    Nucleic Acids Research
    |January 1, 1977
    PubMed
    Summary

    Reovirus messenger RNA (mRNA) 5' termini show variations. Analysis revealed specific methylated cap structures with repeating nucleotide units, indicating sequence divergence.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    ARHI (DIRAS3)-mediated autophagy-associated cell death enhances chemosensitivity to cisplatin in ovarian cancer cell lines and xenografts.

    Cell death & disease·2015
    Same author

    Percutaneous removal of pulmonary artery emboli with Hydrolyser catheter in pigs.

    Canadian Association of Radiologists journal = Journal l'Association canadienne des radiologistes·2001
    Same author

    mRNA 5'-leader trans-splicing in the chordates.

    Genes & development·2001
    Same author

    Human cytomegalovirus IE1 promoter/enhancer drives variable gene expression in all fiber types in transgenic mouse skeletal muscle.

    BMC genetics·2000
    Same author

    Skeletal muscle gene transfer: regeneration-associated deregulation of fast troponin I fiber type specificity.

    American journal of physiology. Cell physiology·2000
    Same author

    A plan that establishes a framework for achieving mutual recognition of good manufacturing practices inspections.

    Food and drug law journal·1999

    Area of Science:

    • Molecular Biology
    • Virology
    • Biochemistry

    Background:

    • Reovirus messenger RNA (mRNA) features a methylated 5' cap structure crucial for translation.
    • Understanding mRNA 5' termini is key to deciphering viral gene expression and regulation.

    Purpose of the Study:

    • To investigate the nucleotide sequences at the 5' termini of reovirus mRNA.
    • To characterize the structure of methylated mRNA synthesized in vitro.

    Main Methods:

    • In vitro synthesis of methylated reovirus mRNA.
    • Ribonuclease T1 digestion of the synthesized mRNA.
    • Analysis of 5' terminal fragments using chain length determination.

    Main Results:

    • Seven distinct 5' terminal fragments were identified.
    • The general structure of these fragments was determined as m7G5'pp5'GmpCpUp(Np)nGp.
    • Chain length analysis indicated that 'n' could be 3, 4, or 5, signifying repeating nucleotide units.

    Conclusions:

    • Reovirus mRNA 5' termini exhibit sequence divergence.
    • The identified fragments provide a detailed structural basis for this divergence.
    • These findings contribute to understanding reovirus mRNA processing and translation initiation.

    Related Experiment Videos