Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The distance between bacterial species in sequence space

R P Ambler1

  • 1Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR Scotland.

Journal of Molecular Evolution
|June 1, 1996
PubMed
Summary

Bacterial species are classified as "tight" or "loose" based on amino acid sequence variation in electron transport proteins. Interspecies differences are significantly larger than intraspecies variation for soil and water bacteria.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[10] Enzymatic hydrolysis with carboxypeptidases.

Methods in enzymology·2012
Same author

[21] carboxypeptidases a and B.

Methods in enzymology·2012
Same author

An alternative to the accepted phylogeny of purple bacteria based on 16S rRNA: analyses of the amino acid sequences of cytochromes C2 and C556 from Rhodobacter (Rhodovulum) sulfidophilus.

Archives of biochemistry and biophysics·2001
Same author

Amino acid sequences of two high-potential iron-sulfur proteins (HiPIPs) from the moderately halophilic purple phototrophic bacterium, Rhodospirillum salinarum.

Archives of biochemistry and biophysics·1999
Same author

Evidence for two distinct azurins in Alcaligenes xylosoxidans (NCIMB 11015): potential electron donors to nitrite reductase.

Biochemistry·1995
Same author

Amino acid sequences of cytochromes c2 and c' from the moderately halophilic purple phototrophic bacterium Rhodospirillum salexigens.

Biochimie·1994

Area of Science:

  • Microbiology
  • Molecular Biology
  • Bioinformatics

Background:

  • Bacterial classification traditionally relies on genus and species, despite advances in macromolecular sequencing.
  • Understanding the genetic diversity within and between bacterial species is crucial for accurate taxonomy.

Purpose of the Study:

  • To compare inter- and intraspecies amino acid sequence differences in bacterial electron transport proteins.
  • To investigate the relationship between sequence variation and the definition of bacterial species.

Main Methods:

  • Analysis of amino acid sequences of cytochromes c and blue copper proteins from various soil and water bacteria.
  • Classification of bacterial species as 'tight' (low intraspecies variation) or 'loose' (high intraspecies variation).

Main Results:

  • Interspecies variation in amino acid sequences was substantially larger than intraspecies variation.
  • Identified 'tight' species (e.g., Pseudomonas aeruginosa) and 'loose' species (e.g., Pseudomonas stutzeri).

Conclusions:

  • Bacterial species exhibit varying degrees of genetic diversity, influencing taxonomic classification.
  • The findings contribute to understanding bacterial evolution and genome distribution in 'sequence space'.

Related Experiment Videos