Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Alternatively spliced transcripts from the Drosophila eIF4E gene produce two different Cap-binding proteins

C A Lavoie1, P E Lachance, N Sonenberg

  • 1Department of Biology, McGill University, Montréal, Québec, Canada H3A 1B1.

The Journal of Biological Chemistry
|July 5, 1996
PubMed
Summary

Researchers identified two distinct cap-binding proteins, eIF4EI and eIF4EII, in Drosophila. These isoforms arise from alternative splicing of a single gene, suggesting complex translational regulation of gene expression.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The SπRIT time projection chamber.

The Review of scientific instruments·2021
Same author

Probing the Symmetry Energy with the Spectral Pion Ratio.

Physical review letters·2021
Same author

The Undiagnosed Diseases Network International: Five years and more!

Molecular genetics and metabolism·2020
Same author

The intra-tumor heterogeneity of cell signaling factors in breast cancer: p4E-BP1 and peIF4E are diffusely expressed and are real potential targets.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2014
Same author

Phosphorylation of eIF4E promotes EMT and metastasis via translational control of SNAIL and MMP-3.

Oncogene·2014
Same author

The ShcA adaptor activates AKT signaling to potentiate breast tumor angiogenesis by stimulating VEGF mRNA translation in a 4E-BP-dependent manner.

Oncogene·2014

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Eukaryotic initiation factor 4E (eIF4E) is crucial for translation initiation, binding mRNA's 5' cap structure.
  • eIF4E is a key regulator of translational control.

Purpose of the Study:

  • To identify and characterize cap-binding proteins in Drosophila.
  • To investigate the molecular basis for different eIF4E isoforms.

Main Methods:

  • 7-methyl-GTP-Sepharose affinity chromatography for protein purification.
  • SDS-polyacrylamide gel electrophoresis for protein size analysis.
  • Peptide microsequence analysis and cDNA cloning for protein identification.

Main Results:

  • Two distinct cap-binding proteins (approx. 35 kDa) were purified from Drosophila adults.

Related Experiment Videos

  • These proteins, eIF4EI and eIF4EII, differ at their amino termini.
  • Analysis revealed three alternatively spliced transcripts from a single eIF4E gene (region 67A8-B2).
  • The transcripts exhibit significant variation in 5'-UTR lengths.

    • Conclusions:

    • Drosophila eIF4E exists as two isoforms, eIF4EI and eIF4EII, generated by alternative splicing.
    • The differential 5'-UTR lengths suggest complex translational control mechanisms regulate eIF4E isoform expression.