Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Saturable ethanol binding in rat liver microsomes

S Channareddy1, S S Jose, V A Eryomin

  • 1Department of Pathology, Medical College of Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.

The Journal of Biological Chemistry
|July 26, 1996
PubMed
Summary

Ethanol binding to liver microsomes is saturable at clinical levels, unlike phospholipids. This suggests specific binding sites in tissues, indicating greater ethanol specificity than previously understood.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Machine-learning-based risk assessment tool to rule out empirical use of ESBL-targeted therapy in endemic areas.

The Journal of hospital infection·2024
Same author

Real-world prevalence across 159 872 patients with cancer supports the clinical utility of TMB-H to define metastatic solid tumors for treatment with pembrolizumab.

Annals of oncology : official journal of the European Society for Medical Oncology·2021
Same author

Comment.

Statistics in biopharmaceutical research·2018
Same author

Systemic chromosome instability in Shugoshin-1 mice resulted in compromised glutathione pathway, activation of Wnt signaling and defects in immune system in the lung.

Oncogenesis·2016
Same author

Challenges in initiating and conducting personalized cancer therapy trials: perspectives from WINTHER, a Worldwide Innovative Network (WIN) Consortium trial.

Annals of oncology : official journal of the European Society for Medical Oncology·2015
Same author

A phase 2 study of MK-0457 in patients with BCR-ABL T315I mutant chronic myelogenous leukemia and philadelphia chromosome-positive acute lymphoblastic leukemia.

Blood cancer journal·2014

Area of Science:

  • Biochemistry
  • Pharmacology
  • Toxicology

Background:

  • Ethanol (alcohol) is widely consumed, and its interactions with biological systems are of significant interest.
  • Understanding ethanol's tissue specificity is crucial for comprehending its physiological and toxicological effects.

Purpose of the Study:

  • To investigate the saturable binding of ethanol to rat liver microsomes.
  • To determine if this binding is specific to microsomal components or due to phospholipids.
  • To characterize the binding sites for ethanol in liver microsomes.

Main Methods:

  • Studying ethanol binding kinetics to isolated rat liver microsomes.
  • Comparing ethanol binding to microsomes versus extracted microsomal phospholipids.
  • Assessing the effect of heat treatment on ethanol binding.

Related Experiment Videos

  • Analyzing binding data to estimate site affinity and capacity.

    • Main Results:

    • Ethanol binding to rat liver microsomes demonstrated saturation at clinically relevant concentrations.
    • No saturable binding was observed in extracted microsomal phospholipids.
    • Heat treatment eliminated saturable ethanol binding.
    • Data analysis suggested at least two types of specific ethanol binding sites: high-capacity and low-capacity.

    Conclusions:

    • The liver microsome's saturable ethanol binding is likely mediated by specific protein sites, not just phospholipids.
    • These findings indicate a higher degree of tissue specificity for ethanol than previously recognized.
    • This specificity may play a role in ethanol's distribution and effects in the body.